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phosphorylated ampk p ampk  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phosphorylated ampk p ampk
    Phosphorylated Ampk P Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+ampk/10__1097_slash_cm9__0000000000004003-33-4-10
    Average 86 stars, based on 1 article reviews
    phosphorylated ampk p ampk - by Bioz Stars, 2026-10
    86/100 stars

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    other:

    Article Title: β-Catenin and AMPK/AKT/FOXO Signaling Mediate Doxorubicin-Induced Senescence and Lipid Accumulation in C2C12 Myoblasts.
    Article Snippet: Antibodies against myogenin (MyoG), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and SREBP-1 were obtained from Santa Cruz Biotechnology (Dallas, TX, USA).

    SDS Page:

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma
    Article Snippet: Total protein was measured using a Pierce TM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12 and 20 μg of protein was resolved by SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% non-fat milk for 75 min. Blots were incubated overnight at 4 °C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynamin-related protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1 S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTOR S2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1 S65 (9451, Cell Signaling Technology). ..

    Membrane:

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma
    Article Snippet: Total protein was measured using a Pierce TM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12 and 20 μg of protein was resolved by SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% non-fat milk for 75 min. Blots were incubated overnight at 4 °C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynamin-related protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1 S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTOR S2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1 S65 (9451, Cell Signaling Technology). ..

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma.
    Article Snippet: Total protein was measured using a PierceTM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12-20 μg of protein was resolved by SDSPAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% nonfat milk for 75 min. Blots were incubated overnight at 4°C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynaminrelated protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTORS2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1S65 (9451, Cell Signaling Technology). ..

    Staining:

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma
    Article Snippet: Total protein was measured using a Pierce TM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12 and 20 μg of protein was resolved by SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% non-fat milk for 75 min. Blots were incubated overnight at 4 °C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynamin-related protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1 S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTOR S2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1 S65 (9451, Cell Signaling Technology). ..

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma.
    Article Snippet: Total protein was measured using a PierceTM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12-20 μg of protein was resolved by SDSPAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% nonfat milk for 75 min. Blots were incubated overnight at 4°C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynaminrelated protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTORS2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1S65 (9451, Cell Signaling Technology). ..

    Incubation:

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma
    Article Snippet: Total protein was measured using a Pierce TM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12 and 20 μg of protein was resolved by SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% non-fat milk for 75 min. Blots were incubated overnight at 4 °C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynamin-related protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1 S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTOR S2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1 S65 (9451, Cell Signaling Technology). ..

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma.
    Article Snippet: Total protein was measured using a PierceTM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12-20 μg of protein was resolved by SDSPAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% nonfat milk for 75 min. Blots were incubated overnight at 4°C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynaminrelated protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTORS2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1S65 (9451, Cell Signaling Technology). ..

    Western Blot:

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma
    Article Snippet: Total protein was measured using a Pierce TM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12 and 20 μg of protein was resolved by SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% non-fat milk for 75 min. Blots were incubated overnight at 4 °C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynamin-related protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1 S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTOR S2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1 S65 (9451, Cell Signaling Technology). ..

    Article Title: Defective autophagy and AMPK inactivation drive ferroptosis in diabetic kidney disease
    Article Snippet: .. Antibodies against the following proteins were used in this study: low density lipoprotein receptor-related protein 2 (LRP2/MEGALIN; a generous gift from T. Michigami, Department of Bone and Mineral Research, Osaka Medical Center and Research Institute for Maternal and Child Health, Japan), sequestosome 1 (SQSTM1/p62; Medical and Biological Laboratory [PM045] for western blotting; Progen [GP62C] for immunostaining), ATG5 (Medical and Biological Laboratory; PM050), microtubule-associated protein light chain 3 (MAP1LC3; Cell Signaling Technology; 2755), β-actin (ACTB; Sigma-Aldrich; A5316), 4-hydroxynonenal (4HNE; Japan Institute for the Control of Aging; MHN-020P), hepatitis A virus cellular receptor 1 (HAVCR1/KIM1; R&D Systems; AF1750), glutathione peroxidase 4 (GPX4) (Abcam; ab125066), acyl-CoA synthetase 4 (ACSL4) (Abcam; ab155282), phosphorylated AMPK (p-AMPK; Cell Signaling Technology; 2535), AMPK (Cell Signaling Technology; 2532), phosphorylated ACC (p-ACC; Cell Signaling Technology; 3661), ACC (Cell Signaling Technology; 3662). .. The biotinylated secondary antibodies were anti-rabbit IgG (BA-1000), anti-mouse IgG (BA-2001), anti-goat IgG (BA-5000) and anti-guinea pig IgG (BA-7000) (all Vector Laboratories).

    Article Title: Defective autophagy and AMPK inactivation drive ferroptosis in diabetic kidney disease.
    Article Snippet: .. Antibodies and reagents Antibodies against the following proteins were used in this study: low density lipoprotein receptor-related protein 2 (LRP2/MEGALIN; a generous gift from T. Michigami, Department of Bone and Mineral Research, Osaka Medical Center and Research Institute for Maternal and Child Health, Japan), sequestosome 1 (SQSTM1/p62; Medical and Biological Laboratory [PM045] for western blotting; Progen [GP62C] for immunostaining), ATG5 (Medical and Biological Laboratory; PM050), microtubule-associated protein light chain 3 (MAP1LC3; Cell Signaling Technology; 2755), β-actin (ACTB; SigmaAldrich; A5316), 4-hydroxynonenal (4HNE; Japan Institute for the Control of Aging; MHN-020P), hepatitis A virus cellular receptor 1 (HAVCR1/KIM1; R&D Systems; AF1750), glutathione peroxidase 4 (GPX4) (Abcam; ab125066), acylCoA synthetase 4 (ACSL4) (Abcam; ab155282), phosphorylated AMPK (p-AMPK; Cell Signaling Technology; 2535), AMPK (Cell Signaling Technology; 2532), phosphorylated ACC (p-ACC; Cell Signaling Technology; 3661), ACC (Cell Signaling Technology; 3662). .. The biotinylated secondary antibodies were anti-rabbit IgG (BA-1000), antimouse IgG (BA-2001), anti-goat IgG (BA-5000) and antiguinea pig IgG (BA-7000) (all Vector Laboratories).

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma.
    Article Snippet: Total protein was measured using a PierceTM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12-20 μg of protein was resolved by SDSPAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% nonfat milk for 75 min. Blots were incubated overnight at 4°C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynaminrelated protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTORS2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1S65 (9451, Cell Signaling Technology). ..

    Binding Assay:

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma
    Article Snippet: Total protein was measured using a Pierce TM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12 and 20 μg of protein was resolved by SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% non-fat milk for 75 min. Blots were incubated overnight at 4 °C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynamin-related protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1 S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTOR S2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1 S65 (9451, Cell Signaling Technology). ..

    Article Title: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma.
    Article Snippet: Total protein was measured using a PierceTM BCA Protein Assay Kit (23225, Thermo Fisher Scientific). .. Between 12-20 μg of protein was resolved by SDSPAGE (Bio-Rad Laboratories, Hercules, CA, USA), transferred onto nitrocellulose membrane or methanol-activated polyvinylidene fluoride (PVDF), normalized using Ponceau S staining solution (40000279, Thermo Fisher Scientific), cut to appropriate size, and blocked in 5% nonfat milk for 75 min. Blots were incubated overnight at 4°C with total OXPHOS rodent WB antibody cocktail (ab110413, Abcam), voltage-dependent anion channel (VDAC, 4866, Cell Signaling Technology, Danvers, MA, USA), peroxin-5 (PEX5, 83020, Cell Signaling Technology), glucose transporter 1 (Glut 1, 12939, Cell Signaling Technology), transketolase (TKT, 64414, Cell Signaling Technology), phosphoenolpyruvate carboxykinase 1 (PCK1, 12940, Cell Signaling Technology), enolase 1 (ENO1, 3810, Cell Signaling Technology), aldolase a (ALDOA, 8060, Cell Signaling Technology), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 2118S, Cell Signaling Technology), phosphoglycerate mutase (PGAM1, Cell Signaling Technology), superoxide dismutase 2 (SOD2, 13194, Cell Signaling Technology), dynaminrelated protein 1 (DRP1, 8570, Cell Signaling Technology), mitochondrial fission factor (MFF, 84580, Cell Signaling Technology), optic atrophy 1 (OPA1, 80471, Cell Signaling Technology), mitofusin 1 (MFN1, 14739, Cell Signaling Technology), mitofusin 2 (MFN2, 9482, Cell Signaling Technology), Parkin (4211, Cell Signaling Technology), liver kinase B1 (LKB1, 3047, Cell Signaling Technology), phosphorylated LKB1S428 (3482, Cell Signaling Technology), adenosine monophosphate activated protein kinase (AMPK, 2532, Cell Signaling Technology), phosphorylated AMPK (2535, Cell Signaling Technology), mammalian target of rapamycin (mTOR, 2983, Cell Signaling Technology), phosphorylated mTORS2448 (2971, Cell Signaling Technology), eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1, 9452, Cell Signaling Technology), or phosphorylated 4E-BP1S65 (9451, Cell Signaling Technology). ..

    Immunostaining:

    Article Title: Defective autophagy and AMPK inactivation drive ferroptosis in diabetic kidney disease
    Article Snippet: .. Antibodies against the following proteins were used in this study: low density lipoprotein receptor-related protein 2 (LRP2/MEGALIN; a generous gift from T. Michigami, Department of Bone and Mineral Research, Osaka Medical Center and Research Institute for Maternal and Child Health, Japan), sequestosome 1 (SQSTM1/p62; Medical and Biological Laboratory [PM045] for western blotting; Progen [GP62C] for immunostaining), ATG5 (Medical and Biological Laboratory; PM050), microtubule-associated protein light chain 3 (MAP1LC3; Cell Signaling Technology; 2755), β-actin (ACTB; Sigma-Aldrich; A5316), 4-hydroxynonenal (4HNE; Japan Institute for the Control of Aging; MHN-020P), hepatitis A virus cellular receptor 1 (HAVCR1/KIM1; R&D Systems; AF1750), glutathione peroxidase 4 (GPX4) (Abcam; ab125066), acyl-CoA synthetase 4 (ACSL4) (Abcam; ab155282), phosphorylated AMPK (p-AMPK; Cell Signaling Technology; 2535), AMPK (Cell Signaling Technology; 2532), phosphorylated ACC (p-ACC; Cell Signaling Technology; 3661), ACC (Cell Signaling Technology; 3662). .. The biotinylated secondary antibodies were anti-rabbit IgG (BA-1000), anti-mouse IgG (BA-2001), anti-goat IgG (BA-5000) and anti-guinea pig IgG (BA-7000) (all Vector Laboratories).

    Article Title: Defective autophagy and AMPK inactivation drive ferroptosis in diabetic kidney disease.
    Article Snippet: .. Antibodies and reagents Antibodies against the following proteins were used in this study: low density lipoprotein receptor-related protein 2 (LRP2/MEGALIN; a generous gift from T. Michigami, Department of Bone and Mineral Research, Osaka Medical Center and Research Institute for Maternal and Child Health, Japan), sequestosome 1 (SQSTM1/p62; Medical and Biological Laboratory [PM045] for western blotting; Progen [GP62C] for immunostaining), ATG5 (Medical and Biological Laboratory; PM050), microtubule-associated protein light chain 3 (MAP1LC3; Cell Signaling Technology; 2755), β-actin (ACTB; SigmaAldrich; A5316), 4-hydroxynonenal (4HNE; Japan Institute for the Control of Aging; MHN-020P), hepatitis A virus cellular receptor 1 (HAVCR1/KIM1; R&D Systems; AF1750), glutathione peroxidase 4 (GPX4) (Abcam; ab125066), acylCoA synthetase 4 (ACSL4) (Abcam; ab155282), phosphorylated AMPK (p-AMPK; Cell Signaling Technology; 2535), AMPK (Cell Signaling Technology; 2532), phosphorylated ACC (p-ACC; Cell Signaling Technology; 3661), ACC (Cell Signaling Technology; 3662). .. The biotinylated secondary antibodies were anti-rabbit IgG (BA-1000), antimouse IgG (BA-2001), anti-goat IgG (BA-5000) and antiguinea pig IgG (BA-7000) (all Vector Laboratories).

    Control:

    Article Title: Defective autophagy and AMPK inactivation drive ferroptosis in diabetic kidney disease
    Article Snippet: .. Antibodies against the following proteins were used in this study: low density lipoprotein receptor-related protein 2 (LRP2/MEGALIN; a generous gift from T. Michigami, Department of Bone and Mineral Research, Osaka Medical Center and Research Institute for Maternal and Child Health, Japan), sequestosome 1 (SQSTM1/p62; Medical and Biological Laboratory [PM045] for western blotting; Progen [GP62C] for immunostaining), ATG5 (Medical and Biological Laboratory; PM050), microtubule-associated protein light chain 3 (MAP1LC3; Cell Signaling Technology; 2755), β-actin (ACTB; Sigma-Aldrich; A5316), 4-hydroxynonenal (4HNE; Japan Institute for the Control of Aging; MHN-020P), hepatitis A virus cellular receptor 1 (HAVCR1/KIM1; R&D Systems; AF1750), glutathione peroxidase 4 (GPX4) (Abcam; ab125066), acyl-CoA synthetase 4 (ACSL4) (Abcam; ab155282), phosphorylated AMPK (p-AMPK; Cell Signaling Technology; 2535), AMPK (Cell Signaling Technology; 2532), phosphorylated ACC (p-ACC; Cell Signaling Technology; 3661), ACC (Cell Signaling Technology; 3662). .. The biotinylated secondary antibodies were anti-rabbit IgG (BA-1000), anti-mouse IgG (BA-2001), anti-goat IgG (BA-5000) and anti-guinea pig IgG (BA-7000) (all Vector Laboratories).

    Article Title: Defective autophagy and AMPK inactivation drive ferroptosis in diabetic kidney disease.
    Article Snippet: .. Antibodies and reagents Antibodies against the following proteins were used in this study: low density lipoprotein receptor-related protein 2 (LRP2/MEGALIN; a generous gift from T. Michigami, Department of Bone and Mineral Research, Osaka Medical Center and Research Institute for Maternal and Child Health, Japan), sequestosome 1 (SQSTM1/p62; Medical and Biological Laboratory [PM045] for western blotting; Progen [GP62C] for immunostaining), ATG5 (Medical and Biological Laboratory; PM050), microtubule-associated protein light chain 3 (MAP1LC3; Cell Signaling Technology; 2755), β-actin (ACTB; SigmaAldrich; A5316), 4-hydroxynonenal (4HNE; Japan Institute for the Control of Aging; MHN-020P), hepatitis A virus cellular receptor 1 (HAVCR1/KIM1; R&D Systems; AF1750), glutathione peroxidase 4 (GPX4) (Abcam; ab125066), acylCoA synthetase 4 (ACSL4) (Abcam; ab155282), phosphorylated AMPK (p-AMPK; Cell Signaling Technology; 2535), AMPK (Cell Signaling Technology; 2532), phosphorylated ACC (p-ACC; Cell Signaling Technology; 3661), ACC (Cell Signaling Technology; 3662). .. The biotinylated secondary antibodies were anti-rabbit IgG (BA-1000), antimouse IgG (BA-2001), anti-goat IgG (BA-5000) and antiguinea pig IgG (BA-7000) (all Vector Laboratories).

    Virus:

    Article Title: Defective autophagy and AMPK inactivation drive ferroptosis in diabetic kidney disease
    Article Snippet: .. Antibodies against the following proteins were used in this study: low density lipoprotein receptor-related protein 2 (LRP2/MEGALIN; a generous gift from T. Michigami, Department of Bone and Mineral Research, Osaka Medical Center and Research Institute for Maternal and Child Health, Japan), sequestosome 1 (SQSTM1/p62; Medical and Biological Laboratory [PM045] for western blotting; Progen [GP62C] for immunostaining), ATG5 (Medical and Biological Laboratory; PM050), microtubule-associated protein light chain 3 (MAP1LC3; Cell Signaling Technology; 2755), β-actin (ACTB; Sigma-Aldrich; A5316), 4-hydroxynonenal (4HNE; Japan Institute for the Control of Aging; MHN-020P), hepatitis A virus cellular receptor 1 (HAVCR1/KIM1; R&D Systems; AF1750), glutathione peroxidase 4 (GPX4) (Abcam; ab125066), acyl-CoA synthetase 4 (ACSL4) (Abcam; ab155282), phosphorylated AMPK (p-AMPK; Cell Signaling Technology; 2535), AMPK (Cell Signaling Technology; 2532), phosphorylated ACC (p-ACC; Cell Signaling Technology; 3661), ACC (Cell Signaling Technology; 3662). .. The biotinylated secondary antibodies were anti-rabbit IgG (BA-1000), anti-mouse IgG (BA-2001), anti-goat IgG (BA-5000) and anti-guinea pig IgG (BA-7000) (all Vector Laboratories).

    Article Title: Defective autophagy and AMPK inactivation drive ferroptosis in diabetic kidney disease.
    Article Snippet: .. Antibodies and reagents Antibodies against the following proteins were used in this study: low density lipoprotein receptor-related protein 2 (LRP2/MEGALIN; a generous gift from T. Michigami, Department of Bone and Mineral Research, Osaka Medical Center and Research Institute for Maternal and Child Health, Japan), sequestosome 1 (SQSTM1/p62; Medical and Biological Laboratory [PM045] for western blotting; Progen [GP62C] for immunostaining), ATG5 (Medical and Biological Laboratory; PM050), microtubule-associated protein light chain 3 (MAP1LC3; Cell Signaling Technology; 2755), β-actin (ACTB; SigmaAldrich; A5316), 4-hydroxynonenal (4HNE; Japan Institute for the Control of Aging; MHN-020P), hepatitis A virus cellular receptor 1 (HAVCR1/KIM1; R&D Systems; AF1750), glutathione peroxidase 4 (GPX4) (Abcam; ab125066), acylCoA synthetase 4 (ACSL4) (Abcam; ab155282), phosphorylated AMPK (p-AMPK; Cell Signaling Technology; 2535), AMPK (Cell Signaling Technology; 2532), phosphorylated ACC (p-ACC; Cell Signaling Technology; 3661), ACC (Cell Signaling Technology; 3662). .. The biotinylated secondary antibodies were anti-rabbit IgG (BA-1000), antimouse IgG (BA-2001), anti-goat IgG (BA-5000) and antiguinea pig IgG (BA-7000) (all Vector Laboratories).



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    Image Search Results


    Effects of corin overexpression on OGD/H-induced mitochondrial dysfunction in HAPI microglia. Following 24 h pre-transduction with corin overexpression or blank lentivirus, cells underwent 24 h OGD/H exposure. Measurements included (A) OCR, (B-F) Mitochondrial complex activities (I, II, III, IV, V), (G) ATP generation, (H) p-AMPK/AMPK protein levels. Data presented as mean ± SD (n = 3). All experiments were repeated three times. Significant differences between groups were assessed using one-way ANOVA with Tukey’s post-hoc test. ***P < 0.001 vs. control. # P < 0.05, ## P < 0.01, ### P < 0.001 vs. OGD/H＋vector.

    Journal: BMB Reports

    Article Title: Corin inhibits microglial inflammatory activation by suppressing mitochondrial dysfunction in intracerebral hemorrhage

    doi: 10.5483/BMBRep.2025-0175

    Figure Lengend Snippet: Effects of corin overexpression on OGD/H-induced mitochondrial dysfunction in HAPI microglia. Following 24 h pre-transduction with corin overexpression or blank lentivirus, cells underwent 24 h OGD/H exposure. Measurements included (A) OCR, (B-F) Mitochondrial complex activities (I, II, III, IV, V), (G) ATP generation, (H) p-AMPK/AMPK protein levels. Data presented as mean ± SD (n = 3). All experiments were repeated three times. Significant differences between groups were assessed using one-way ANOVA with Tukey’s post-hoc test. ***P < 0.001 vs. control. # P < 0.05, ## P < 0.01, ### P < 0.001 vs. OGD/H＋vector.

    Article Snippet: Membranes were incubated overnight at 4°C with primary antibodies targeting Corin (Proteintech, 83566-1-RR), Iba-1 (Proteintech, 81728-1-RR), phosphorylated AMPK (p-AMPK; Abclonal, AP1002), AMPK (Proteintech, 10929-2-AP), and β-actin (Proteintech, 66009-1-Ig).

    Techniques: Over Expression, Transduction, Control

    Overexpression of corin mitigates OGD/H-induced cell migration impairment, inflammatory response, and mitochondrial dysfunction in HAPI microglia via the AMPK pathway. Cells were pre-transduced with corin-overexpressing or control lentivirus for 24 h, followed by OGD/H and treatment with 10-μM Compound C for 24 h. Subsequent assessments included: (A) cell viability, (B) migration, (C) iNOS and Arg-1 mRNA levels, (D) Iba-1, p-AMPK, and AMPK protein levels, (E) OCR, (F-J) activities of mitochondrial complexes I-V, and (K) ATP generation. Data presented as mean ± SD (n = 3). All experiments were repeated three times. Significant differences between groups were assessed using one-way ANOVA with Tukey’s post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001 vs. OGD/H＋vector＋vehicle. # P < 0.05, ## P < 0.01, ### P < 0.001 vs. OGD/H＋Corin+vehicle.

    Journal: BMB Reports

    Article Title: Corin inhibits microglial inflammatory activation by suppressing mitochondrial dysfunction in intracerebral hemorrhage

    doi: 10.5483/BMBRep.2025-0175

    Figure Lengend Snippet: Overexpression of corin mitigates OGD/H-induced cell migration impairment, inflammatory response, and mitochondrial dysfunction in HAPI microglia via the AMPK pathway. Cells were pre-transduced with corin-overexpressing or control lentivirus for 24 h, followed by OGD/H and treatment with 10-μM Compound C for 24 h. Subsequent assessments included: (A) cell viability, (B) migration, (C) iNOS and Arg-1 mRNA levels, (D) Iba-1, p-AMPK, and AMPK protein levels, (E) OCR, (F-J) activities of mitochondrial complexes I-V, and (K) ATP generation. Data presented as mean ± SD (n = 3). All experiments were repeated three times. Significant differences between groups were assessed using one-way ANOVA with Tukey’s post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001 vs. OGD/H＋vector＋vehicle. # P < 0.05, ## P < 0.01, ### P < 0.001 vs. OGD/H＋Corin+vehicle.

    Article Snippet: Membranes were incubated overnight at 4°C with primary antibodies targeting Corin (Proteintech, 83566-1-RR), Iba-1 (Proteintech, 81728-1-RR), phosphorylated AMPK (p-AMPK; Abclonal, AP1002), AMPK (Proteintech, 10929-2-AP), and β-actin (Proteintech, 66009-1-Ig).

    Techniques: Over Expression, Migration, Transduction, Control

    Sodium-glucose co-transporter-2 inhibitor- and progranulin knockout-induced autophagy via the mechanistic target of rapamycin complex 1 pathway in the kidneys of each group. Western blot analysis of the protein levels of (A) phosphorylated AMP-activated protein kinase (p-AMPK) and AMPK, (B) p-Akt and Akt, and (C) p-S6 and S6 in the kidneys. Comparisons among groups were performed with one-way analysis of variance followed by Tukey tests. Data are expressed as mean±standard deviation. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; WT, wild-type; DM, diabetes mellitus; Tofo, tofogliflozin; KO, knockout. a P <0.05, b P <0.01 vs. WT-DM; c P <0.05 vs. WT-Tofo.

    Journal: Endocrinology and Metabolism

    Article Title: Effects of Progranulin Deficiency on Inflammation and Fibrosis in the Kidneys and Liver of Diabetic Mice Fed a High-Fat Diet

    doi: 10.3803/EnM.2025.2339

    Figure Lengend Snippet: Sodium-glucose co-transporter-2 inhibitor- and progranulin knockout-induced autophagy via the mechanistic target of rapamycin complex 1 pathway in the kidneys of each group. Western blot analysis of the protein levels of (A) phosphorylated AMP-activated protein kinase (p-AMPK) and AMPK, (B) p-Akt and Akt, and (C) p-S6 and S6 in the kidneys. Comparisons among groups were performed with one-way analysis of variance followed by Tukey tests. Data are expressed as mean±standard deviation. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; WT, wild-type; DM, diabetes mellitus; Tofo, tofogliflozin; KO, knockout. a P <0.05, b P <0.01 vs. WT-DM; c P <0.05 vs. WT-Tofo.

    Article Snippet: Membranes were blocked with a blocking reagent (0.1% Tween 20 and 5% bovine serum albumin in Tris-buffered saline) for 1 hour and subsequently incubated overnight at 4°C with the following primary antibodies (1:1,000): phospho-S6 (#2211; Cell Signaling Technology, Danvers, MA, USA), S6 (#2217; Cell Signaling Technology), phosphorylated AMP-activated protein kinase (p-AMPK) (#2531; Cell Signaling Technology), AMPK (#2532; Cell Signaling Technology), phospho-Akt (#4060; Cell Signaling Technology), Akt (#4691; Cell Signaling Technology), peroxisome proliferator-activated receptor α (PPARα) (#SC-398394; Santa Cruz Biotechnology, Dallas, TX, USA), and PPARγ (#2435; Cell Signaling Technology).

    Techniques: Knock-Out, Western Blot, Standard Deviation

    Sodium-glucose co-transporter-2 inhibitor- and progranulin knockout-induced autophagy via the mechanistic target of rapamycin complex 1 pathway in the livers of each group. Western blot analysis of the protein levels of (A) phosphorylated AMP-activated protein kinase (p-AMPK) and AMPK, (B) p-Akt and Akt, and (C) p-S6 and S6 in the liver. Comparisons among groups were performed with one-way analysis of variance followed by Tukey tests. Data are expressed as mean±standard deviation. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; WT, wild-type; DM, diabetes mellitus; Tofo, tofogliflozin; KO, knockout. a P <0.05.

    Journal: Endocrinology and Metabolism

    Article Title: Effects of Progranulin Deficiency on Inflammation and Fibrosis in the Kidneys and Liver of Diabetic Mice Fed a High-Fat Diet

    doi: 10.3803/EnM.2025.2339

    Figure Lengend Snippet: Sodium-glucose co-transporter-2 inhibitor- and progranulin knockout-induced autophagy via the mechanistic target of rapamycin complex 1 pathway in the livers of each group. Western blot analysis of the protein levels of (A) phosphorylated AMP-activated protein kinase (p-AMPK) and AMPK, (B) p-Akt and Akt, and (C) p-S6 and S6 in the liver. Comparisons among groups were performed with one-way analysis of variance followed by Tukey tests. Data are expressed as mean±standard deviation. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; WT, wild-type; DM, diabetes mellitus; Tofo, tofogliflozin; KO, knockout. a P <0.05.

    Article Snippet: Membranes were blocked with a blocking reagent (0.1% Tween 20 and 5% bovine serum albumin in Tris-buffered saline) for 1 hour and subsequently incubated overnight at 4°C with the following primary antibodies (1:1,000): phospho-S6 (#2211; Cell Signaling Technology, Danvers, MA, USA), S6 (#2217; Cell Signaling Technology), phosphorylated AMP-activated protein kinase (p-AMPK) (#2531; Cell Signaling Technology), AMPK (#2532; Cell Signaling Technology), phospho-Akt (#4060; Cell Signaling Technology), Akt (#4691; Cell Signaling Technology), peroxisome proliferator-activated receptor α (PPARα) (#SC-398394; Santa Cruz Biotechnology, Dallas, TX, USA), and PPARγ (#2435; Cell Signaling Technology).

    Techniques: Knock-Out, Western Blot, Standard Deviation